Journal: Heliyon

Article Title: Influence of iron binding in the structural stability and cellular internalization of bovine lactoferrin

doi: 10.1016/j.heliyon.2021.e08087

Figure Lengend Snippet: Internalization of bLf in Vero cells. BLf in its apo (A) or holo (B) forms were conjugated to FITC and synchronously incubated with Vero cells for 0, 30 or 60 min. BLf internalization was observed by laser-scanning confocal fluorescence microscopy. The selected groups of cells are representative of their respective open fields. White bars: 20 μm.

Article Snippet: Apo- and holo-bLf were incubated with FITC (Molecular Probes, Eugene, OR, USA) at a molar ratio of 1:10 in basic phosphate buffer (2.5% Na 2 HPO 4 .7H 2 O and 0.082% NaH 2 PO 4 at pH 8.0) for 1 h at 4 °C.

Techniques: Incubation, Fluorescence, Microscopy

Journal: Developmental biology

Article Title: Developmental Guidance of Embryonic Corneal Innervation: Roles of Semaphorin3A and Slit2

doi: 10.1016/j.ydbio.2010.04.032

Figure Lengend Snippet: Anterior eyes from embryonic ages E4-E7 as brightfield images – overlayed by fluorescent images showing nerves (arrows) labeled red with the TuJ-1 antibody and growing toward the cornea. In E4 the cornea is designated with a “C”. At E7 arrowheads designate small nerve branches growing towards the cornea, but not into it. The scale in all figures is the same, as shown by the bar with the E4 image, which is 1mm.

Article Snippet: Then, to visualize axons, the cultures were fixed in 4% paraformaldehyde and immunolabeled with the TuJ-1 antibody followed by donkey anti-mouse Northern lights Red fluorescent secondary antibody (R&D Systems) Images were captured using a fluorescent stereo dissecting microscope (SMZ 1500, Nikon Instruments, Melville, NY) equipped with a SPOT Flex real time CCD camera (Diagnostic Instruments, Inc., Sterling Heights, MI) and neuron numbers and lengths were analyzed using ImageJ software.

Techniques: Labeling

Journal: Developmental biology

Article Title: Developmental Guidance of Embryonic Corneal Innervation: Roles of Semaphorin3A and Slit2

doi: 10.1016/j.ydbio.2010.04.032

Figure Lengend Snippet: (A and B) Fluorescent images of E7 TuJ-1 labeled OTG co-cultured with an E7 lens “L” in the presence of (A) 10μg/ml rabbit IgG antibody or (B) 10μg/ml Slit2 antibody. (C) Neurite numbers and (D) neurite lengths from OTG co-cultured with lens or cornea and treated with 10μg/ml of either Slit2 antibody or control rabbit IgG. Neurite numbers (E) and neurite lengths (F) from co-cultures of E7 OTG with E7 cornea treated with 10μg/ml rabbit IgG antibody, 10μg/ml Slit2 antibody, 3μg/ml recombinant mouse Slit2, or 10μg/ml Slit2 antibody and 3μg/ml recombinant mouse Slit2. Error bars in C - F show the SEM. Scale bar in B = 0.3mm.

Article Snippet: Then, to visualize axons, the cultures were fixed in 4% paraformaldehyde and immunolabeled with the TuJ-1 antibody followed by donkey anti-mouse Northern lights Red fluorescent secondary antibody (R&D Systems) Images were captured using a fluorescent stereo dissecting microscope (SMZ 1500, Nikon Instruments, Melville, NY) equipped with a SPOT Flex real time CCD camera (Diagnostic Instruments, Inc., Sterling Heights, MI) and neuron numbers and lengths were analyzed using ImageJ software.

Techniques: Labeling, Cell Culture, Control, Recombinant

Journal: The European respiratory journal

Article Title: Classical protein kinase C and its hypoxic stimulus-induced translocation in the cat and rat carotid body.

doi: 10.1034/j.1399-3003.2000.016003459.x

Figure Lengend Snippet: Fig. 1. ± Confocal fluorescence microscopy of double immunolabelling of a section of a normoxic cat carotid body tissue stained with mouse anti- protein kinase C (PKC)c and rabbit anti-tyrosine hydroxylase (TH). The localization of PKCc is visualized in green with fluorescein isothiocyanate labelled antimouse immunoglobulin G (IgG) (A), whereas that of TH in red with Texas red-labelled antirabbit IgG (B). An overlapping image is shown in C; the yellow spots indicate colocalization of the two fluorescent probes. The near-perfect colocalization identifies the PKCc as being in the chemoreceptor cell. (Internal scale bars=10 mm.)

Article Snippet: For immunofluorescence staining, sections were incubated with fluorescein isothiocyanate (FITC)-labelled antimouse immunoglobulin G (IgG; dilution 1:200; Jackson Immunoresearch West Grove, PA, USA) for 1 h. To confirm the chemoreceptor cell localization of the PKC antigens, other sections were double-labelled by simultaneous incubation with a rabbit polyclonal antibody directed against rat tyrosine hydroxylase (TH; dilution 1:500; Chemicon International, Inc., Temecula, CA, USA) and visualization with Texas red-labelled antirabbit IgG (dilution 1:200; Jackson Immunoresearch).

Techniques: Fluorescence, Microscopy, Staining